Article 1418

Title of the article

PROSPECTS OF USING DILUTED MEDIUM FOR CYANOBACTERIA RECULTIVATION 

Authors

Petrukhina Dar'ya Igorevna, Applicant, Tsiolkovsky Kaluga State University (26 Stepana Razina street, Kaluga, Russia),
E-mail: daria.petrukhina@outlook.com 

Index UDK

606:582.232 

DOI

10.21685/2307-9150-2018-4-1 

Abstract

Background. Spirulina and Arthrospira species include a wide variety of laboratory cyanobacteria culture collections and their cryo-storage does provide long-term preservation. The aim of the work is to study a possibility of cultivation in diluted Zarrouk medium of the Spirulina and Arthrospira collection (9 strains) after 12 months of cryopreservation at a temperature of minus 80 °C.
Materials and methods. The effluent of Zarrouk medium was obtained from cyanobacteria’s Spirulina and Arthrospira cultivation. Biomasses of Spirulina and Arthrospira were subject to cryopreservation. The effluent of Zarrouk medium was mixed (1:1) with new Zarrouk medium to reuse after cryo-storage.
Results. The article presents data concerning the influence of diluted Zarrouk medium on growth rate of Spirulina and Arthrospira after cryo-storage. The growth of these cyanobacteria after thawing was satisfactory and featured the same rate as the growth in the new Zarrouk medium.
Conclusions. The results of this work shows that reuse of Zarrouk medium may be appropriate to recultivation of the cyanobacteria Spirulina and Arthrospira collection after long-term cryo-storage. 

Key words

Arthrospira sp., Spirulina sp., culture medium, reuse of Zarrouk medium 

 

 Download PDF

References

1. Eriksen N. T. Applied Microbiology and Biotechnology. 2008, vol. 80, iss. 1, pp. 1–14. DOI 10.1007/s00253-008-1542-y.
2. Vonshak A. Spirulina platensis (Arthrospira): physiology, cell-biology and biotechnology. London: CRC Press, 2002, 252 p.
3. Sili C., Torzillo G., Vonshak A. Ecology of Cyanobacteria II: Their Diversity in Space and Time. 2012, pp. 677–705. DOI 10.1007/978-94-007-3855-3_25.
4. Zarrouk C. Contribution à l’étude d’une cyanophycée: influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de Spirulina maxima (Setch et Gardner) Geitler: Ph. D. thesis [Contribution to the study of cyanophyceae: influence
of various physical and chemical factors on growth and photosynthesis of Spirulina maxima (Setch and Gardner) Geitler: Ph. D. thesis]. Paris: Université de Paris, 1966, 109 p.
5. Loftus S. E., Johnson Z. I. Algal Research. 2017, vol. 24, part A, pp. 154–166. DOI 10.1016/j.algal.2017.03.007.
6. Morocho-Jácome A. L., Mascioli G. F., Sato S., de Carvalho J. C. M. Algal Research. 2016, vol. 13, pp. 159–166. DOI 10.1016/j.algal.2015.11.022.
7. Morocho-Jácome A. L., Sato S., de Carvalho J. C. M. Journal of Chemical Technology & Biotechnology. 2015, vol. 91, iss. 4, pp. 901–910. DOI 10.1002/jctb.4655.
8. Petrukhina D. I., Lykov I. N. Izvestiya vuzov. Prikladnaya khimiya i biotekhnologiya [University proceedings. Applied chemistry and biotechnology]. 2016, vol. 6, no. 4, pp. 68–73. DOI 0.21285/2227-2925-2016-6-4-68-73.
9. Petrukhina D. I., Lykov I. N. Tekhnologii zhivykh system [Living system technologies]. 2018, vol. 15, no. 1, pp. 49–54. 

 

Дата создания: 22.03.2019 08:35
Дата обновления: 22.03.2019 10:14