Article 1418

Title of the article



Petrukhina Dar'ya Igorevna, Applicant, Tsiolkovsky Kaluga State University (26 Stepana Razina street, Kaluga, Russia),

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Background. Spirulina and Arthrospira species include a wide variety of laboratory cyanobacteria culture collections and their cryo-storage does provide long-term preservation. The aim of the work is to study a possibility of cultivation in diluted Zarrouk medium of the Spirulina and Arthrospira collection (9 strains) after 12 months of cryopreservation at a temperature of minus 80 °C.
Materials and methods. The effluent of Zarrouk medium was obtained from cyanobacteria’s Spirulina and Arthrospira cultivation. Biomasses of Spirulina and Arthrospira were subject to cryopreservation. The effluent of Zarrouk medium was mixed (1:1) with new Zarrouk medium to reuse after cryo-storage.
Results. The article presents data concerning the influence of diluted Zarrouk medium on growth rate of Spirulina and Arthrospira after cryo-storage. The growth of these cyanobacteria after thawing was satisfactory and featured the same rate as the growth in the new Zarrouk medium.
Conclusions. The results of this work shows that reuse of Zarrouk medium may be appropriate to recultivation of the cyanobacteria Spirulina and Arthrospira collection after long-term cryo-storage. 

Key words

Arthrospira sp., Spirulina sp., culture medium, reuse of Zarrouk medium 


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Дата создания: 22.03.2019 08:35
Дата обновления: 22.03.2019 10:14